pM220


Vector IG Sequence Link :
General : plasmid ds-DNA 8200 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli RR1)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Contains EcoRI, PstI, HindIII, BamHI and BglII sites. A yeast/E.coli shuttle expression vector containing the Saccharomyces cerevisiae TPI promoter fused to the signal sequence for MFalpha1 (yeast mating pheromone alpha-factor). Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 1.8, 1.3; HindIII--8.2; BamHI--8.2. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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