Vector IG Sequence Link :
General : phagemid ds-DNA 4152 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli AN1459)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Constructed by replacing the 1.118 kb BglI fragment of pCE30 with the analogous 1.27 kb fragment of pTZ18U. Upon infection with M13KO7 (ATCC 37468), an M13-sensitive host strain containing a pMA200U derivative produces packaged single-stranded plasmid DNA complementary to the M13 universal primer. If the insert is oriented correctly for transcription from the lambda promoters, the single-stranded plasmid DNA will contain the coding strand. Restriction digests of the clone give the following sizes (kb): BamHI--4.3; BamHI/EcoRI--4.3; BglI--3.0, 1.3. (ATCC staff) Expression vector encoding cI857. Contains primer sites useful for sequencing and permits production of ssDNA. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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