Vector IG Sequence Link :
General : plasmid ds-DNA 3803 BP
Functions : (cloning)(mutagenesis site-directed)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Corrected 20-DEC-1986 by J. Thierack, MPI Biochemie, Martinsried Corrected 10-JUL-1987 by P. Stanssens and F. Pfeiffer Vector for multiple rounds of oligonucleotide-directed construction of mutations. One derivative (pMA5) confers ampicillin resistance, the other (pMC5) confers resistance to chloramphenicol. Using specific oligonucleotides, resistance is shifted together with site-directed mutagenesis between these two resistance modes. The PstI site in the polylinker region is not unique. Presence of CAA at pos 2811-13 was shown by DNA sequencing. It is unknown, if this also applies to the parent sequence (pBR325)!
Parents : (PF1)(pBR325)(Poly8)(EcoTrp)
Siblings : ()
Descendents : (pMAC5bg14)(pMAC7-8)


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