Vector IG Sequence Link :
General : plasmid ds-DNA 4354 BP
Functions : (protein design)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : (ATCC)
Misc.Comments : Expression vector permitting production of a fusion protein and containing signal sequences for protein export. Also permits production of ssDNA and contains priming sites useful for sequencing. Encodes the ompA leader. Contains a chloramphenical acetyltransferase gene with an amber mutation useful for gapped-duplex mutagenesis experiments. Expression can be induced by a temperature shift in hosts providing a temperature-sensitive lambda repressor, or by batch transduction in a growing culture to repressor-negative hosts. The order of the major features in this phagemid is:lambdaPL promoter - ompA leader - NaeI site - MCS - priming site - fd ori - pMB1 ori - bla - cat (am). Restriction digests of the clone give the following sizes (kb): SalI--4.3; PstI--2.3, 2.0; XbaI--4.3; EcoRI--4.3; HindIII--4.3. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : (pBR325)(pMAC5-8)
Siblings : ()
Descendents : ()


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