pMB


Vector IG Sequence Link :
General : plasmid ds-DNA 5590 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(E.coli JM105)(E.coli JM109)(vertebrate cells)
Suppliers : (ATCC)
Misc.Comments : Deposited by: J. Justin McCormick The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. [1] Both sites permit detection of inserts by alpha complementation, with discrimination between the two provided by the amber mutation in the second lacZ. [1] Expression vector containing two lacZalpha-based multiple cloning sites, with the second containing an amber mutation and being flanked by an RSV enhancer-MMTVLTR promoter at one end and splicing and polyadenylation signals at the other. [1] The plasmid contains the following restriction sites (bp from position 0): NotI--0; EcoRI--9, 3071; ClaI--425, 1560, 1745; Drd--1398, 3583, 5452; PvuI--1596, 2953, 4741; BglII--1782; SphI--2907, 3110; NsiI--2909; HindIII--99, 1794, 3112; BsaI--4441. (personal communication) The order of the major features in this plasmid is: NotI - 5' RSV enhancer - MMTV LTR 3' - ClaI - 5' lacZ'amb5 - NruI/MCSII''/HindIII - 3' lacZ'amb5 - splicing - polyadenylation - NsiI - 3' lacZ' EcoRI/MCS/HindIII - 5' lacZ'- pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--2.5, 1.8, 1.3; EcoRI--3.0, 2.6; HindIII--2.6, 1.7, 1.3; BglII--5.6; ClaI--4.3, 1.3. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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