pMFalpha8


Vector IG Sequence Link :
General : plasmid ds-DNA 7000 BP
Functions : (cloning)(shuttle)(expression)
Selection : ()
Copy Number :
Hosts : (E.coli MC1061)(Saccharomyces cerevisiae)(E.coli)(E.coli HB101)
Suppliers : (ATCC)(DNAX Research Institute)
Misc.Comments : This was constructed by inserting a modified alpha promoter leader fragment into pTRP584, in which the StuI site in the TRP1-ARS1 fragment is replaced with a PvuII site, so that the StuI site in the alpha factor processing region is unique. This is a Saccharomyces cerevisiae/E.coli shuttle vector with regulatory sequences to permit processing and secretion of eukaryotic proteins. The cloning sites are just downstream of the first processing site for the alpha factor leader. Shuttle expression vector permitting processing and secretion of eukaryotic proteins. Cloning sites are immediately downstream of the first processing site for the alpha factor leader. Constructed by inserting a modified alpha promoter leader sequence into pTRP584, replacing the StuI site in the TRP1-ARS1 fragment with a PvuII site, so that the StuI site in the alpha factor processing region is unique. The order of the major features in this plasmid is: 2 micron ori - alpha factor leader - StuI - XhoI - TRP5 - EcoRI - pMB1 ori - ampR - EcoRI - ARS1 - PvuII - TRP1 - EcoRI. [1] Note: This material is cited in a U.S and/or other Patent and may not be used to infringe the patent claims. Medium is 1227 LB plus ampicillin. Deposited by: DNAX Research Inst. of Molecular and Cell. Biol,, Inc.
Parents : ()
Siblings : ()
Descendents : ()


Return to Vector Homepage