Vector IG Sequence Link :
General : plasmid ds-DNA 6200 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.8 doublet, 0.87; EcoRI--3.8, 2.8; BamHI--6.4. (ATCC staff) The insert contains bp -109 to +105 of the MMTV promoter; the 5' boundary is a natural SstI site. [1] There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts. (personal communication) This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California.(personal communication) One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells. [1] Medium is 1227 LB plus ampicillin.
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