Vector IG Sequence Link :
General : plasmid ds-DNA 9064 BP
Functions : (cloning)
Selection : (color blue/white)
Copy Number : low
Hosts : (E.coli TB1)(E.coli DH5alphaF')(E.coli)(Gram-positive bacteria)(broad host range)
Suppliers : (ATCC)
Misc.Comments : Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.8; SspI--4.9, 1.8, 1.6, 1.0; EcoRI/MluI--8.2, 0.9. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : (pMMB66EH)
Descendents : (pMMB206)


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