Vector IG Sequence Link :
General : plasmid ds-DNA 9311 BP
Functions : (cloning)
Selection : (color blue/white)
Copy Number : low
Hosts : (E.coli TB1)(E.coli DH5alphaF')(E.coli)(Gram-positive bacteria)(broad host range)
Suppliers : (ATCC)
Misc.Comments : Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction. [1] Sequencing demonstrated that there are 3 stop codons in frame with the truncated bla gene, terminating translation but permitting continued transcription and expression of the cat gene. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--9.3; EcoRV--5.8, 3.6; SspI--3.9, 1.8, 1.6, 1.2, 0.9; EcoRI/MluI--8.4, 0.9. (ATCC staff) Medium is 1675 LB plus chloramphenicol (10 ug/ml).
Parents : (pMMB190)
Siblings : (pMMB66EH)
Descendents : ()


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