pMMB277


Vector IG Sequence Link :
General : plasmid ds-DNA 9237 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli TB1)(broad host range)(E.coli)(E.coli DH5alphaF')
Suppliers : (ATCC)
Misc.Comments : Constructed by replacing the MluI/EcoRI fragment of pMMB206 (taclacUV5 promoter) with the MluI/EcoRI fragment from pMMB311 (tac promoter). Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; EcoRV--4.2, 1.7, 1.5, 1.1, 0.9; EcoRI/MluI--8.4, 0.8. (ATCC staff) Medium is 1675 LB plus chloramphenicol (10 ug/ml). NCBI gi: 209093
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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