pMMB66HE


Vector IG Sequence Link :
General : plasmid ds-DNA 8821 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(broad host range)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Restriction digests of the clone give the following sizes (kb): AccI--3.8, 3.1, 1.9; BamHI--8.6; HindIII/PvuII--7.4, 1.3. (ATCC staff) Contains the following sites separated by (bp): EcoRI - 739 - AhaIII - 97 - ScaI - 112 - PvuI - 2101 - AccI - 1848 - ScaI - 55 - AccI - 2039 - BstEII - 372 - PvuII - 93 - PvuII - 94 - HpaI - 320 - BstEII - 182 - MluI - 771 - EcoRI. (personal communication) The sequence of RSF1010 is given in this reference. [2] A 1246 bp lacIQ HindIII fragment inserted at XmaIII/BamHI of pKK223-3. A M13mp9 polylinker replaced the M13mp8 polylinker of pMMB66EH (ATCC 37620). THe 3005 bp NruI/AhaIII fragment was cloned between PstI (7768) and PvuII (1951) of RSF1010. [1] In the pMMB66 (ATCC 37620, 37621) and pMMB67 (ATCC 37622, 37623) vector series, the EH and HE in the name designate the orientation of the multiple cloning site to the tac promoter. (personal communication) An autoregulated high-level expression vector utilizing the tac promoter, rrnB terminators and lacIq for lac repression. Concentration of induced gene product may be regulated by varying the lac inducer concentration. [1] Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. [1] Medium is 1227 LB plus ampicillin.
Parents : (pMMB66EH)(pJF118HE)
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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