pMSC1


Vector IG Sequence Link :
General : plasmid ds-DNA 13100 BP
Functions : (cloning)(shuttle)(library)
Selection : ()
Copy Number :
Hosts : (Mycobacterium)(E.coli)
Suppliers : (ATCC)
Misc.Comments : A cosmid shuttle vector useful for genomic DNA library construction. The ScaI site between the cos sites can be used to linearize the plasmid. After packaging, 10.3 kb of vector is retained, permitting an insert range of 30 - 42 kb. Sequences hybridizing to pBR322, including ampR, are removed. The pAL5000 ori permits replication in Mycobacterium smegmatis and M. bovis BCG. The order of the major features in this plasmid is: cos - pMB1 ori - ampR/ScaI - cos - trpA terminator - XbaI - HindIII - fd terminator - EcoRV - pAL5000 ori - BamHI - rrnC terminator - EcoRV - lambda ori - NheI - kanR. [1] Growth media: 1065 + ampicillin (50 ug/ml) + kanamycin (20 ug/ml) 37C Deposited by: N.G.Stoker
Parents : ()
Siblings : ()
Descendents : ()


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