Vector IG Sequence Link :
General : plasmid ds-DNA 5400 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Kanamycin resistance should be checked at regular intervals. This is best done during amplification steps using LB medium supplemented with 50 ug/ml ampicillin and 12.5 ug/ml kanamycin. (personal communication) The SV40 promoter has a 3 bp deletion at the BglI site within the origin of replication to prevent autonomous replication in the presence of SV40 large T-antigen. The MSV LTR provides a polyadenylation signal as well as an enhancer for the P1/thymidine kinase promoter regulating the Tn5 kanR/G418R transcription unit. A BamHI/HindIII digest gives fragments of 3.0 and 2.5 kb. Restriction digest of the clone give the following sizes (kb): HindIII--5.9; BamHI--5.9; EcoRI--5.9; BamHI/HindIII--3.2, 2.7; BglII--5.9; consistent with deposited DNA. (ATCC staff) The order of the major features in this plasmid is:SV40 promoter - MSV-LTR - P1 promoter - TK promoter - kanR/G418R - TK polyadenylation signal - ampR. Shuttle expression vector. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pUC19)(pHS272)(pMSPE)
Siblings : ()
Descendents : ()

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