pMTchi5/4269C


Vector IG Sequence Link :
General : plasmid ds-DNA 10800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli LE392)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : This vector contains the following sites separated by (kb): BamHI - 0.15 - PvuII - 0.33 - BamHI - 9.2 - PvuII - 1.6 - BamHI. A 480 bp HindIII/Sau3AI fragment from pTiA6 was isolated, the Sau3AI site changed to BamHI and the ends filled. BamHI linkers were added and it was cloned into the BamHI site of MTX5. An episomal Saccharomyces cerevisiae/E.coli shuttle vector. Restriction digests of the clone give the following sizes (kb): HindIII--11.3; PvuII--9.6, 2.0; BamHI--11.3. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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