pMTVCAT)(GMCAT


Vector IG Sequence Link :
General : plasmid ds-DNA 6893 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)(E.coli K-12)(monkey COS-1 cell line [ATCC CRL 1650])
Suppliers : (ATCC)
Misc.Comments : Plasmid to be cotransfected with plasmids encoding steroid receptors such as pRShGR (ATCC 67200) and pRShMR (ATCC 67201) to provide a reporter gene for hormone-dependent transcriptional activation. [3] Contains the LTR from mouse mammary tumor virus (nt -142 to nt -109), sequences encoding chloramphenicol acetyltransferase, and SV40 splicing sequences. [3] Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 1.8; PvuII--5.95, 0.3; BglI--4.8, 1.2; HindIII--3.55, 2.45; PstI--6.0. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (MTV LTR)(
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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