pNASSbeta


Vector IG Sequence Link :
General : plasmid ds-DNA 6541 BP
Functions : (expression)(reporter)(promoter analysis)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(E.coli)(monkey COS cells)(vertebrate cells)
Suppliers : (CLONTECH)(ATCC)
Misc.Comments : ATCC size is 6700 bp. The order of the major features in this plasmid is: pUC19 - EcoRI - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. [1] The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Promoter-less reporter plasmid permitting visual detection of transcription/translation activity by histochemical staining. [1] Promoters may be introduced upstream of the lacZ cassette at the unique EcoRI site. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--6.7; HindIII--6.7; EcoRI/HindIII--4.0, 2.7; NotI--3.5, 3.2; BamHI--3.65, 2.85, 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml).
Parents : (pBR322)
Siblings : (pADbeta)(pCMVbeta)(pSVbeta)(pTKbeta)
Descendents : ()


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