pNF2


Vector IG Sequence Link :
General : plasmid ds-DNA 7800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(Rhodobacter)
Suppliers : (ATCC)
Misc.Comments : The spcR marker is expressed in R. capsulatus. A wide range of transcription levels can be obtained by varying the nitrogen source in the medium. Transcription can be immediately stopped by the introduction of oxygen and ammonia. A Rhodobacter capsulatus expression vector with transcription of the insert under the control of the nifH promoter. Restriction digests of the clone give the following sizes (kb): SalI--8.2; BamHI--8.2; EcoRI/PstI--4.2, 3.6. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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