pNH218


Vector IG Sequence Link :
General : plasmid ds-DNA 11500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (Bacillus subtilis 168)(Bacillus subtilis)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--7.8, 3.8; HindIII--6.8, 4.7. (ATCC staff) A 1.1 kb EcoRI fragment with Bacillus licheniformis alpha-amylase regulatory & signal sequences was cloned into pUC9 cut with EcoRI/SmaI. A 3.0 kb BamHI/HindIII fragment (E.coli phoA minus signal sequences and the first 5-13 aa) was inserted. pBs81/6 (Bacillus replication) was cut with HindIII and cloned into the HindIII site. An E.coli/Bacillus spp. shuttle expression vector for the construction and secretion of fusion proteins. Contains the alkaline phosphatase gene of E.coli. The plasmid appears to be unstable. We recommend that the ATCC-provided stock be streaked for single colonies and that a colony with the correct restriction pattern be selected. (ATCC staff) Medium is 1065 LB medium.
Parents : ()
Siblings : ()
Descendents : ()


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