pNKY48


Vector IG Sequence Link :
General : plasmid ds-DNA 4300 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae DBY745)(Saccharomyces cerevisiae SK-1)(Saccharomyces cerevisiae S288C)
Suppliers : (ATCC)
Misc.Comments : Contains a HIS4C/URA3 fusion cassette that can be used to make fusions to other proteins of interest. Also includes a M13 sequencing primer approximately 300 bp from the HIS4C/URA3 fusion junction. Encodes 93 amino acids of HIS4C and the complete 267 amino acids of URA3. Can be used for promoter/enhancer trap screens. (personal communication) Constructed by inserting a 1.4 kb HindIII fragment from pNKY2029 containing the HIS4/URA3 fusion into pNKY47,which is a derivative of pGC-1 with the original HindIII site destroyed & a new one created at the BamHI site of the MCS. The order of the major features in this plasmid is: M13 primer - ClaI - XhoI - SalI - XbaI (stop) - BamHI - HindIII - 90 aa HIS4C bridge/URA3 - BamHI - HindIII - BamHI - BglII - NcoI - EcoRI - M13 ori - pMB1 ori - ampR. Restriction digests of the clone give the following sizes (kb): PstI--4.4; BglII--4.4; HindIII--3.1, 1.3; EcoRI--4.4; XbaI--4.4. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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