pNN414


Vector IG Sequence Link :
General : yeast artificial chromosome ds-DNA 15809 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH1)(Saccharomyces cerevisiae)(vertebrate cells)(E.coli)(Saccharomyces cerevisiae AB1380)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Restriction digests of the clone give the following sizes (kb): EcoRI--15.9; XhoI--11.0, 3.4, 1.0; (ATCC staff) BamHI--14.0, 1.85; BamHI/EcoRI--8.2, 5.8, 1.85; SalI/BamHI--7.4, 6.6, 1.85. (ATCC staff) XhoI linkers were ligated to the blunt ended 4.2 kb PvuI/BamHI fragment of pSV2neo (with neomycin resistance and ColE1 origin). This was then cloned into the SalI site of pYAC4. Contains telomer elements from Tetrahymena. To facilitate chromosome walking, the ends of the insert can be recovered in E.coli. The artificial chromosomal DNA can be digested with XhoI, ligated, and used to transform E.coli to amp or kan resistance. Cloning into the EcoRI site insertionally inactivates SUP4. G418 resistance is expressed in mammalian cell lines to permit genetic complementation experiments. Constructed by Christopher Traver. Medium is 1227 LB plus ampicillin.
Parents : (pSV2-neo)(pYAC4)
Siblings : ()
Descendents : ()


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