pNOT322


Vector IG Sequence Link :
General : plasmid ds-DNA 4369 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alphaF')(E.coli)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. General purpose cloning vector containing a unique NotI site. Constructed for insertion of mobilization cassettes pMOB1-4 (ATCC 77280-77283) to produce a vector useful for allele replacement in Pseudomonas aeruginosa. The depositor requests that this plasmid be available only for non-commercial research purposes. (ATCC staff) Restriction digests of the clone give the following sizes (kb): HindIII--4.37; EcoRI--4.37; EcoRI/BamHI--4.0,0.35; NotI--4.37. (ATCC staff) Medium is 1315 1065 plus ampicillin (100 ug/ul).
Parents : (pBR322)
Siblings : ()
Descendents : ()


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