pOF216


Vector IG Sequence Link :
General : plasmid ds-DNA 12500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli SC1451)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Ligating lambda cosR adapters onto the termini of potential inserts and cosL adapters onto the linearized vector enhances the step of circularization of large molecules. [1] The polylinker is inserted in frame within the lacZ gene facilitating selection for blue and white colonies. [1] This is a low copy number mini-F based plasmid vector with rare restriction sites in its polylinker for cloning inserts of up to 100 kb. [1] The depositor recommends adding spectinomycin to the media at a concentration of 20-30 ug/ml at the first establishment of spcR transformants and 15 ug/ml thereafter. (personal communication) The EcoRI site is available for cloning but the lacPO N-terminal region will be deleted. [1] Restriction digests of the clone give the following sizes (kb): BamHI--12.5; HindIII--12.5. (ATCC staff) Medium is -1 1065 plus spectinomycin (15 ug/ml).
Parents : ()
Siblings : ()
Descendents : ()

NCBI ENTREZ Link :


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