pOJ31


Vector IG Sequence Link :
General : cosmid ds-DNA 5000 BP
Functions : (cloning)(shuttle)
Selection : ()
Copy Number :
Hosts : (E.coli)(actinomycetes)
Suppliers : (ATCC)
Misc.Comments : Medium copy number shuttle vector allowing lacZalpha complementation. Constructed from the cosmid vector pFD304 by addition of a lacZalpha-polylinker fragment and a 2.4 kb PstI-SalI fragment containing the JHJ-3 replicon. The JHJ-3 replicon permits replication in the following actinomycete genera: Streptomyces, Saccharopolyspora and Amycolatopsis. The order of the major features in the plasmid is: JHJ-3 ori - neo - ClaI* - synthetic promoter - pMB1 ori - cos site - lac promoter - SpeI/MCS/XbaI - lacZ. The ClaI* site is methylated, making the ClaI site in the polylinker unique in E. coli dam+ strains. [2] Growth medium: LB plus kanamycin (50 ug/ml)(ATCC number 1236) 37C Deposited by: C.V.Dery
Parents : ()
Siblings : ()
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