pOM2


Vector IG Sequence Link :
General : plasmid ds-DNA 2716 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli)
Suppliers : (ATCC)
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled by F. Pfeiffer, MPI Biochemie, Martinsried Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation. [1] pOM1 (ATCC 77220) and pOM2 (ATCC 77221) differ in the orientation of the polylinker. [1] Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7; XhoI--2.7. (ATCC staff) E.coli transformed with this plasmid produce larger colonies when an insert is present in the polylinker than when there is no insert. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : (M13tg130)(pUC8)(pUC830)(bGal130)
Siblings : (pOM1)(pOM3)(pOM4)(pOM5)(pOM6)(pOM7)(pOM8)
Descendents : ()

NCBI ENTREZ Link :


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