pOM3


Vector IG Sequence Link :
General : plasmid ds-DNA 2713 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli)
Suppliers : (ATCC)
Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled by F. Pfeiffer, MPI Biochemie, Martinsried Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation. [1] pOM3 (ATCC 77222) and pOM4 (ATCC 77223) differ in the orientation of the polylinker. [1] Contains an additional ATG in the inserted ClaI linker in a different reading frame which might also function as start codon for expression. [1] Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (M13tg131)(pUC9)(pUC931)(bGal131)
Siblings : (pOM1)(pOM2)(pOM4)(pOM5)(pOM6)(pOM7)(pOM8)
Descendents : ()

NCBI ENTREZ Link :


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