pPB101


Vector IG Sequence Link :
General : plasmid ds-DNA 12280 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : A plasmid expression vector constructed from spleen necrosis virus, an avian retrovirus. [2] The insert contains an internal SalI site about 860 bp from the EcoRI end. [1] An EcoRI/BglII double digest yields a 2.0 kb fragment containing an LTR and initiator ATG for the gag protein. [1] This was constructed by cloning the 7.7 kb SalI fragment from p60B Sal (containing the SalI fragment of clone 60B) into the SalI site of pSW210 (pBR322 containing the AvrII/SalI fragment of pSNV-60B), thus generating an insert with 2 LTRs. [1] Medium is 1227 LB plus ampicillin.
Parents : (pSW210 from pBR322 and pSNV-60B)(p60B)
Siblings : ()
Descendents : (pPB102)


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