pPGKNeo


Vector IG Sequence Link :
General : plasmid ds-DNA 1 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JA221)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The promoter and terminator sequences were derived from the phosphoglycerate kinase gene. Saccharomyces cerevisiae containing a single copy of the neomycin phosphotransferase gene are sensitive to G418; resistance to 400 ug/ml is expressed if there are several copies per cell. The G418-resistance segment includes a 0.7 kb region of the PGK promoter and a 1.5 kb BglII/BamHI fragment from Tn5. The BamHI site is between the G418R and the PGK terminator. Restriction digests of the clone give the following sizes (kb): BamHI--15.0; BglII--15.0; XhoI--9.4, 6.0; EcoRI--13.0, 2.1; KpnI--13.0, 2.2. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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