pPH3


Vector IG Sequence Link :
General : plasmid ds-DNA 2500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli)(E.coli DH5alphaF')
Suppliers : (ATCC)
Misc.Comments : Expression is repressed in the presence of excess inorganic phosphate (>1%) and high concentrations of glucose (>1%). Maximal induction was observed at limiting inorganic phosphate (<0.1 mM) and normal glucose (0.2-0.4%). The order of the major features in this plasmid is:L1 terminator - ugp promoter - EcoRI - MCS - HindIII - rpoC terminator - ori - bla. The nucleotide sequence of the transcription start point before the MCS is GCGAGCATAA AACGCGT. An expression vector useful in possibly all E.coli strains containing a functional pho regulatory circuit. The insert must supply the ribosome-binding site. Restriction digests of the clone give the following sizes (kb): HindIII--2.6; EcoRI--2.6; HindIII/EcoRI--2.6; BglII--1.8,0.80. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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