Vector IG Sequence Link :
General : plasmid ds-DNA 3716 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli M5219)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The following unique restriction sites are found on this vector separated by (bp)(approx): BglI - 100 - PstI - 750 - EcoRI - 600 - XhoI - 300 - SmaI - 250 - HindIII - 1650. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--3.7; PstI--XhoI--2.3, 1.4; BglI--3.7. (ATCC staff) Sequencing has demonstrated that the OL PL region is unchanged from lambda but that one A at position 41 of the 5' untranslated region of the N gene has been deleted. The translational start for the N gene product is missing. [1] The orientation of the kanR segment in pPLa2311 is such that the HindIII site is closer to the ori-containing fragment. [1] The orientation of the PL promoter is anticlockwise with respect to the plasmid ori. [1] This vector has 3 HaeII fragments: one about 2 kb containing PL and beta-lactamase from pBR322, one from pMK20 carrying kanR, and one 370 bp containing the ori segment from pMK20. [1] pPLa2311 in M5219 does not form colonies at 42C, even in the absence of antibiotic selection. Plating efficiency is variable in K12deltaH1 at 42C. [1] E.coli M5219 is E.coli K-12 M72 lac(am) trp(am) rpsL lambda cI857 deltaH1 bio252. deltaH1 removes part of cro and all genes to the right of cro. bio252 removes all genes to the left of cIII. At 42C, N is expressed from the chromosome. [1] This vector is used for expression of fragments containing a ribosome binding site. [1] Medium is 1227 LB plus ampicillin.
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