pPLa8


Vector IG Sequence Link :
General : plasmid ds-DNA 3716 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli deltaH1)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The following unique restriction sites are found on this vector separated by (bp)(approx): BamHI - 900 - EcoRI - 600 - XhoI - 300 - SmaI - 250 - HindIII - 1650. [1] Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--3.0, 0.7; BglI/XhoI--2.2, 1.5; SmaI--3.7; ClaI--3.7; HindIII--3.7. (ATCC staff) This vector was derived from pPLa2311 (ATCC 31694) by using linkers to convert the PstI site in the beta-lactamase gene to a BamHI site. The Ala at position 182 is replaced with Arg-Ile-Arg & beta-lactamase activity is lost. [1] The orientation of the PL promoter is anticlockwise with respect to the plasmid ori. [1] E.coli K-12 deltaH1 is K-12 M72 lac(am) deltatrpEA2 rpsL lambda cI857 N(am7)N(am53) deltaH1 bio-. deltaH1 removes part of cro & all genes to the right of cro. [1] This vector is used for expression of fragments containing a ribosome binding site. [1] Medium is 1236 LB plus kanamycin.
Parents : ()
Siblings : ()
Descendents : ()

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