pPLc28


Vector IG Sequence Link :
General : plasmid ds-DNA 2720 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli deltaH1)(E.coli RR1)(E.coli)
Suppliers : (ATCC)
Misc.Comments : The following unique restriction sites are found on this vector separated by (bp) (approx): BglI - 100 - PstI - 150 - PvuI - 1020 - EcoRI - 10 - BamHI - HindIII - 1420. [1] The orientation of the PL promoter is clockwise with respect to the plasmid ori. [1] The vectors pPLc28 (ATCC 31696) and pPLc24 (ATCC 31697) with the PL promoter oriented clockwise relative to the plasmid origin of replication plated showed reduced plating efficiency in E.coli M5219 at 42C under antibiotic selection. [1] The vectors pPLc28 (ATCC 31696) and pPLc24 (ATCC 31697) with the PL promoter oriented clockwise relative to the plasmid origin of replication plated equally well at 28C and 42C when in E.coli K-12 deltaH1 hosts. [1] E.coli K-12 deltaH1 is K-12 M72 lac(am) deltatrpEA2 rpsL lambda cI857 N(am7)N(am53) deltaH1 bio-. deltaH1 removes part of cro & all genes to the right of cro. [1] This vector is used for expression of fragments containing a ribosome binding site. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; PstI--2.7; BglI--2.7; HindIII--2.7; BamHI--2.7. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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