pPM1


Vector IG Sequence Link :
General : plasmid ds-DNA 3600 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (depositor-supplied DNA)(E.coli)
Suppliers : (ATCC)
Misc.Comments : This was constructed by inserting a 0.9 kb ClaI-BamHI fragment from pCQV2 (containing lambdaPR & cI857) into M13mp9 (AccI/BamHI). This was used to make PstI/SmaI fragment with the SmaI site adjacent to the PR initiation site and inserted into pUC9. [2] This vector contains the cI857 temperature sensitive mutant gene of the lambda cI repressor. Temperature control can be used to repress, in vivo, transcription of potentially deleterious genes during culture in E.coli. [1] The unique EcoRI site in the vector occurs downstream of an insert into the SmaI site. Plasmid with insert can be linearized with EcoRI to generate a transcript. [1] In some cases, significant yields of transcript initiated at the first nucleotide of inserted 5'-pyrimidine-terminated DNA fragment can also be obtained using this vector. [4] Transcription will be initiated at the first nucleotide of any 5'-purine-terminated DNA fragment fused to the SmaI site. [4] The PR promoter in this vector has been modified to position the cleavage site of a unique SmaI site exactly at the initiation site of the PR promoter. [4] This is a vector for in vitro transcription from a modified lambda PR promoter. [4] Distributed in aliquots of 187.5 ng (3.75 ul, 50 ng/ul). (ATCC staff) Medium is 1227 LB plus ampicillin.
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