Vector IG Sequence Link :
General : plasmid ds-DNA 8800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JM109)(broad host range)(E.coli)(Pseudomonas aeruginosa PAO1)(E.coli K-12)
Suppliers : (ATCC)
Misc.Comments : Promoter-cloning shuttle vector permitting visual detection of activity from divergent promoters. Uses the promoterless luciferase gene from Vibrio harveyi (luxAB) and the promoterless alkaline phosphatase (phoA) as the reporter groups. The order of the major features in this plasmid is: ori(pMB1) - ori(1600) - ampR - phoA - Shine-Dalgarno sequence (SD) - translational stop codons - multiple cloning site - tsc - SD - luxAB. phoA and luxAB are oriented divergently, with the HindIII end of the MCS closer to luxAB. Restriction digests of the clone give the following sizes (kb): PstI--5.6, 3.2; HindIII--8.8; BamHI--8.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()

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