pQF60


Vector IG Sequence Link :
General : plasmid ds-DNA 5800 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli JM109)(broad host range)(E.coli)(Pseudomonas aeruginosa PAO1)(E.coli K-12)
Suppliers : (ATCC)
Misc.Comments : Promoter-cloning shuttle vector permitting visual detection of activity. Uses the promoterless alkaline phosphatase gene (phoA) as the reporter group. The order of the major features in this plasmid is: ori(pMB1) - ori(1600) - ampR - terminator - multiple cloning site - phoA. Translational stop codons in all reading frames are located between the MCS and the translational initiation codon of the reporter gene. Restriction digests of the clone give the following sizes (kb): HindIII--5.8; BamHI--5.8; EcoRI--4.6, 1.2; XbaI--5.8. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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