Vector IG Sequence Link :
General : plasmid ds-DNA 11400 BP
Functions : (cloning)(integrating)(shuttle)
Selection : ()
Copy Number :
Hosts : (Saccharomyces cerevisiae)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Homologous recombination between pRAN4 and a clone in pYAC4 results in replacement of sequences in the right arm of pYAC4 with those of pRAN4 encoding ampR, G418R, and ADE2. The 1.8 kb BamHI-EcoRI fragment of pRAN4 is homologous to the SmaI-ApaI fragment of pYAC4. Transform S. cerevisiae containing YAC clones with BamHI-linearized pRAN4 and select for recombinants that grow in the absence of adenine (Ura- Ade+ Trp+). Maintenance of the E. coli ori, ampR and kanR genes allows plasmid rescue of YAC insert ends. Restriction sites available for right-end plasmid rescue include XhoI, XbaI, BglII and HindIII. The order of the major features of the plasmid is: BamHI - 1.8 kb pYAC4 homologous sequence - EcoRI - pMB1 ori/ampR - HindIII - SV40 ori - BglII - neo (kanR, G418R) - SmaI - ADE2 - EcoRI - HindIII - Tetrahymena telomere. [1] Modification shuttle vector to construct larger yeast artificial chromosomes (YACs) by mitotic recombination. Also facilitates transfer to mammalian cells. [2] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: D.C.Markie
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