Vector IG Sequence Link :
General : plasmid ds-DNA 139 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(Klebsiella pneumoniae)
Suppliers : ()
Misc.Comments : In the absence of the products of nifA, ntrA, or both the transcription start site of the nifH/lacZ gene, with a point mutation in the promoter region, is shifted from the wild-type nifH mRNA start site at position 101 to positions 60-62. However, when the products of nifA and ntrA are both present transcription starting at positions 60-62 is abolished and the normal transcription start site at position 101 is used. In wild type plasmid the upstream start sites at 60-62 are also used in the absence of the nifA and ntrA products. [1] proposes that the point mutation in the nifH/lacZ promoter region in pRB1 may activate a pre-existing, low-efficiency promoter sequence. NCBI gi: 208904
Parents : (Klebsiella pneumoniae)
Siblings : ()
Descendents : ()


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