pRB382


Vector IG Sequence Link :
General : plasmid ds-DNA 8900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(Bacillus subtilis)(B.subtilis DB403)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Reinhold Bruckner Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. [1] Translational fusion shuttle vector using 'lacZ as reporter. Also contains the vegII promoter. Useful for construction of fusion proteins. [1] The 5' truncated 'lacZ gene, lacking transcriptional and translational initiation signals, is useful in both E. coli and in B. subtilis, since the endogenous beta-galactosidase level in B. subtilis is low. [1] The Bacillus subtilis promoter vegII initiates transcription in both B. subtilis and E. coli. [1] Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. [1] May not be suitable for cloning very strong expression signals. [1] The order of the major features in the plasmid is: To terminator - vegII promoter - HindIII/MCS/BamHI - 'lacZ - rrnB terminator - ampR - pMB1 ori - pUB110 ori - neoR - bleR. [1] Restriction digests of the clone give the following sizes (kb): BamHI--9.5; EcoRI/BglI--5.1, 2.2, 1.1, 0.8; BglI/BglII--3.5, 2.2, 2.0, 1.4. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


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