pRB394


Vector IG Sequence Link :
General : plasmid ds-DNA 6500 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(E.coli HB101)(Bacillus subtilis)(B.subtilis DB403)
Suppliers : (ATCC)
Misc.Comments : Deposited by: Reinhold Bruckner Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. [1] Promoter-cloning shuttle vector using the expression of chloramphenicol acetyltransferase (CAT) as the reporter. Also useful for construction of fusion proteins. [1] The cat gene was derived from pUB112 by deletion of the promoter and the regulatory inverted repeat, resulting in constitutive cat gene expression when provided with an appropriate upstream promoter. [1] Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. [1] May not be suitable for cloning very strong expression signals. [1] The order of the major features in the plasmid is: To terminator - HindIII/MCS/EcoRI - cat - rrnB terminator - ampR - pMB1 ori - pUB110 ori - neoR - bleR. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--6.7; BamHI--6.7; BglI/BglII--3.5, 3.2. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


Return to Vector Homepage