pLUS


Vector IG Sequence Link :
General : plasmid ds-DNA 8580 BP
Functions : (cloning)(shuttle)
Selection : ()
Copy Number :
Hosts : (E.coli)(Saccharomyces)
Suppliers : (ATCC)
Misc.Comments : Shuttle vector maintained at approximately 15 copies per haploid genome in S. cerevisiae and 2-3 copies per genome in C. albicans. Recombinants can be detected using blue/white color selection. Transformation of S. cerevisiae AH22 with pRC2312 resulted in replicative transformants at high frequency (1.41(E)5 per ug DNA). Transformants of C. albicans SGY-243 with pRC2312 gave replicative transformants at high frequency (5.42(E)3 per ug DNA) and integrative transformants at lower frequency (32 per ug DNA). Candida albicans genes LEU2 and URA3 were isolated from plasmids pMK155 and pCARS1 respectively. The C. albicans autonomously replicating sequence (CaARS) allows replication in C. albicans and S. cerevisiae. The order of the major features in the plasmid is: ampR - pMB1 ori - lacZ/HindIII/MCS/SmaI - URA3 - CaARS - LEU2. [2] Deposited by: R.D.Cannon Growth media: LB plus ampicillin (ATCC medium number 1227) 37C
Parents : ()
Siblings : ()
Descendents : ()


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