pRD8


Vector IG Sequence Link :
General : plasmid ds-DNA 6300 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)(vertebrate cells)(D17 cell line for infection)(D17-C3 helper cell line for transfection)
Suppliers : (ATCC)
Misc.Comments : The 5' 10 bp of U3 in the left LTR were deleted, so there is no homology between the two U3 sequences and the risk of recombination is reduced. [1] A replication-defective retroviral vector which is promoter-deficient in the right LTR and cannot express retroviral RNA. Foreign gene expression is regulated by the SV40 early promoter. [1] pJD220SvHy (ATCC 67393) and pRD8 (ATCC 67394) differ in the orientation of the SV40 promoter/hygromycin phosphotransferase B sequences. pRD8 has an additional 3' RNA processing sequence between the left U5 and the hygromycin sequences. [1] Removal of SNV map units 7.747 to 8.127 results in deletion of the entire U3 sequence, except for 10 bp ath the 5' end containing the attL site. [1] Constructed from pJD220Hy by inserting a 3' processing sequence between SstI and SalI sites, removing the hygro gene, and replacing it in the opposite orientation with the SV40 promoter. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.6; EcoRI--2.8, 2.0, 1.5; HindIII--uncut; SalI--6.2; XhoI--6.2. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : ()
Siblings : ()
Descendents : ()


Return to Vector Homepage