pRFS10


Vector IG Sequence Link :
General : plasmid ds-DNA 1 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : Hypersynthesis of araC protein has been accomplished by deleting some of the 5' noncoding DNA in front of the araC gene and inserting a 95 bp lac fragment containing the promoter and the ribosome binding site. The level of the araC protein was increased 5000 times over that in the wild type gene. Hypersynthesis, however, does not require the lac ribosome binding site. Apparently a sequence already present in front of the araC gene 'ggag' (base pairs 180-183) can serve as an efficient binding site.
Parents : (pBR322)(paraC116)
Siblings : (pRFS11)
Descendents : (pRFS13)(pRFS20A)(pRFS15)


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