Vector IG Sequence Link :
General : plasmid ds-DNA 202 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : Hypersynthesis of araC protein has been accomplished by deleting some of the 5' noncoding DNA in front of the araC gene and inserting a 95 bp lac fragment containing the promoter and the ribosome binding site. The level of the araC protein was increased 5000 times over that in the wild type gene. Hypersynthesis, however, does not require the lac ribosome binding site. Apparently a sequence already present in front of the araC gene 'ggag' (base pairs 180-183) can serve as an efficient binding site. Clone pRFS20A, another clone that causes hypersynthesis of the araC protein, is missing 17 bp in the lac direction (including the ribosome binding site) and 21 bp in the arac direction. It appears in another entry. NCBI gi: 207980
Parents : (pRFS10)(pRFS11)(pGL101 from pLJ3)
Siblings : (pRFS20A)(pRFS15)
Descendents : ()


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