Vector IG Sequence Link :
General : plasmid ds-DNA 166 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli)
Suppliers : ()
Misc.Comments : Hypersynthesis of araC protein has been accomplished by deleting some of the 5' noncoding DNA in front of the araC gene and inserting a 95 bp lac fragment containing the promoter and the ribosome binding site. The level of the araC protein was increased 5000 times over that in the wild type gene. Hypersynthesis, however, does not require the lac ribosome binding site. Apparently a sequence already present in front of the araC gene 'ggag' (base pairs 144-147) can serve as an efficient binding site. Clone pRFS13, another clone that causes hypersynthesis of the araC protein and includes the lac ribosome binding site, appears in another entry. NCBI gi: 207982
Parents : (pRFS10)(pRFS11)(pGL101 from pLJ3)
Siblings : (pRFS13)(pRFS15)
Descendents : ()


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