Vector IG Sequence Link :
General : plasmid ds-DNA 6230 BP
Functions : (cloning)(promoter detection)
Selection : ()
Copy Number :
Hosts : (E.coli JPA101)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.3; EcoRI--6.2. (ATCC staff) RL724 (ATCC 37683) and RL726 (ATCC 37684) differ in having the M13 intergenic region in opposite orientations in a different size fragment. This plasmid was constructed from the galK leader of pKO100 fused to the lacZ of pMC1403; the M13 intergenic region of pZ152; the multiple cloning sites of M13mp19; and the trp a terminator of pRL118. This is a promoter-cloning vector with beta-galactosidase reporter gene, trp a terminator upstream of MCS,galK leader with stop codons in 3 reading frames, and intergenic region of M13 to permit ssDNA preparation for mutagenesis and/or sequencing. Medium is 1227 LB plus ampicillin. NCBI gi: 209562
Parents : ()
Siblings : ()
Descendents : ()


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