pRS200


Vector IG Sequence Link :
General : phagemid ds-DNA 4793 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Modified from pRS314 (ATCC 77143) by replacing the SmaI site in the MCS with BglII linkers. Permits double digestion of the vector to lower background when inserting Sau3AI fragments. (personal communication) YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: TRP1 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4. An EcoRI site in the TRP1-containing fragment (external to the coding sequence) was destroyed. Restriction digests of the clone give the following sizes (kb): PvuI--2.8, 2.0; HindIII--3.7, 1.1; EcoRI--3.5, 1.3; XbaI--3.4, 1.4; PvuII--4.3; 0.4. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pRS314)
Siblings : ()
Descendents : ()


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