pRS304


Vector IG Sequence Link :
General : phagemid ds-DNA 4267 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae YPH499)(Saccharomyces cerevisiae YPH500)(Saccharomyces cerevisiae YPH501)
Suppliers : (ATCC)
Misc.Comments : One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene. YI-type integrating shuttle vector permitting visual detection of recombinants and production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. The order of the major features in this plasmid is:TRP1 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A genomic HincII/PstI fragment (1.002 kb) containing the TRP1 gene was inserted into the NdeI site of pRSS56. All ends were blunted and an internal EcoRI site was destroyed. Restriction digests of the clone give the following sizes (kb): HindIII--3.3, 0.94; PvuII--2.8, 0.48; XbaI--2.8, 1.4; EcoRI--4.4. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416305
Parents : ()
Siblings : (pRS303)(pRS305)(pRS306)
Descendents : ()

NCBI ENTREZ Link :


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