Vector IG Sequence Link :
General : phagemid ds-DNA 5504 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae YPH499)(Saccharomyces cerevisiae YPH500)(Saccharomyces cerevisiae YPH501)
Suppliers : (ATCC)
Misc.Comments : One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene. The order of the major features in this plasmid is:LEU2 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla. YI-type integrating shuttle vector permitting visual detection of recombinants & production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (2.235 kb) containing the LEU2 gene was inserted into the NdeI site of pRSS56. All ends were blunted. Restriction digests of the clone give the following sizes (kb): HindIII--5.6; PvuII--5.1, 0.46; KpnI--3.8, 1.7; EcoRI--3.5, 2.0. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416306
Parents : ()
Siblings : (pRS304)(pRS303)(pRS306)
Descendents : ()


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