pRS313


Vector IG Sequence Link :
General : phagemid ds-DNA 4967 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)(Saccharomyces cerevisiae YPH499)(Saccharomyces cerevisiae YPH500)(Saccharomyces cerevisiae YPH501)
Suppliers : (ATCC)
Misc.Comments : One of a series of pBluescript-based centromere vectors (ATCC 77142 to ATCC 77145, ATCC 77157 to ATCC 77158) differing in the yeast selectable marker gene. The order of the major features in this plasmid is: HIS3 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4. A fragment (1.184 kb) containing the HIS3 gene was inserted into the NdeI site and a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; BamHI--5.0; PvuII--4.5, 0.48. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416308
Note: there is experimental evidence (restriction digest) that there is a SfiI site in the HIS3 sequence in the pRS series vectors that use HIS3 as a marker. Thus, the sequence matches the genomic version of HIS3 Z75110, not the annotated version X03245 found in Genbank (personal communication, A. Holtz, November 11, 2002). The sequence stored in VectorDB will match X03245 until the Genbank entry is properly updated.
Parents : ()
Siblings : (pRS314)(pRS315)(pRS316)
Descendents : ()

NCBI ENTREZ Link :


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