Vector IG Sequence Link :
General : phagemid ds-DNA 6900 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli DH5alpha)(vertebrate cells)(E.coli)
Suppliers : (ATCC)
Misc.Comments : Constructed by cloning the HindIII/XbaI fragment from pWX008 into pRc/RSV. [1] Transient expression experiments should be performed to examine the RSV promoter efficiency in different cell lines. The RSV promoter can be replaced by excising with a BglII/HindIII digest. (personal communication) Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. [1] The order of the major features in pRc/RSV is: RSV LTR - MCS - bovine growth hormone polyadenylation signal - M13 ori - SV40 early promoter - neoR - SV40 polyadenylation signal - pMB1 ori - bla. [1] Presence of alcohol dehydrogenase activity is used to follow cell lineage in culture or in situ. [1] pRSVlacZII (ATCC 77129), pRSVPAP (ATCC 77130) and pRSVADH (ATCC 77131) provide distinct color-staining reactions (aqua blue, red, and blue-black respectively) to permit simultaneous analysis of multiple lineages. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 2.6; HindIII--6.9; BglII--6.9; KpnI--6.9; XbaI--6.9; HindIII/XbaI--5.1, 1.8. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin.
Parents : (pRcRSV from Invitrogen)(pWX008)
Siblings : (pRSVPAP)(pRSVlacZ)
Descendents : ()

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