Vector IG Sequence Link :
General : plasmid ds-DNA 2853 BP
Functions : (cloning)
Selection : ()
Copy Number :
Hosts : (E.coli HB101)(E.coli CAG-456)(E.coli DH1)
Suppliers : (ATCC)
Misc.Comments : Created by Moore, July 1995, under contract with NCBI. Restriction digests of the clone give the following sizes (kb): EcoRI--2.85; ClaI--2.85. (ATCC staff) When E.coli CAG-456 (a protease deficient host) is used this vector yields large amounts (100 mg/liter or 45% of the soluble protein) of the fusion protein. This vector contains the trpE operon and 51 bp of the trpE gene, followed by a 12-mer EcoRI linker and the M13mp13 multiple cloning site (MCS). There is a TGA stop codon in frame from the GAA of the EcoRI site 33 bp beyond the MSC ClaI site. One of a series of plasmid expression vectors (ATCC 37677 to 37679) representing multiple reading frames for expression from trpE promoter. The fusion proteins produced contain less than 25 amino acids of the trpE protein. Use E.coli SG935 (ATCC 39623), SG936 (ATCC 39624) or WM6 (ATCC 47020) as host for high level production of recombinant fusion protein. (ATCC staff) Medium is 1227 LB plus ampicillin.
Parents : (pATH1)
Siblings : ()
Descendents : ()


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